| IGFBP-7

Insulin-like growth factor-binding protein 7 is a protein that is encoded by the IGFBP7 gene.The major function of the protein is the regulation of availability of insulin-like growth factors (IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with low affinity compared to IGFBPs 1-6.[8][9] It also stimulates cell adhesion. The protein is implicated in some cancers.[The edited region contains a proposed heparin binding site and is also part of the recognition sequence for proteolytic cleavage. Heparin binding inhibits cell binding and cell adhesion functions of the protein.Cleavage which occurs at amino acid position 97 reduces heparin binding but modulates the growth stimulatory activity of the protein.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.04	1.38	0.88	0.53	0.35	0.24	0.18	0.1
Corrected OD	1.94	1.28	0.78	0.43	0.25	0.14	0.08	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human IGFBP-7 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-111	99
Plasma(n=8)	82-107	100
Cell Culture Supernates(n=8)	82-114	99

 

| Linearity

Samples were spiked with high concentrations of Human IGFBP-7 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	82-111	85-104	92-105
	Average (%)	100	96	99
1 : 4	Range (%)	89-108	82-110	84-114
	Average (%)	96	95	99